Device

Part:BBa_I746201:Design

Designed by: Narin Hengrung   Group: iGEM07_Cambridge   (2007-08-31)


Outer Membrane Permeability Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 299
    Illegal AgeI site found at 665
    Illegal AgeI site found at 1292
    Illegal AgeI site found at 1810
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method.


Source

The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba.

References